is the Most Effective Antioxidant during Human Plasma and Blood Ozonization
A. Larini, V. Bocci
The use of a solution of arachidonic acid as the substrate, uric acid, ascorbic acid and human albumin as the antioxidants at physiological concentrations, undergoing either oxygenation or ozonization with ozone concentrations within the previously determined therapeutic range (20-80 µg/ml per ml of solution, corresponding to 0.42-1.67 mM), has emphasized the potent antioxidant properties of albumin. Moreover it has been shown that lipid-bound albumin undergoes peroxidation in sequential steps depending upon progressively increasing ozone concentrations (up to 400 µg/ml per ml of solution, corresponding to 8.33 mM). Using human blood samples, critical erythrocytic enzymes (glutathione peroxidase, glutathione reductase, superoxide dismutase and glucose-6-phosphate dehydrogenase) retain their activity when blood is briefly exposed to ozone concentrations within the therapeutic range. Taken together these data fully confirm the capacity of the antioxidant system to adequately control ozone reactivity.