Ozone Treatment Does Not Induce Toxicity in Human NIH3T3 Fibroblast Cells
D. Amendola, B. Bucci, E. Brunetti, M. Martinelli

SUMMARY - Ozone (O2-O3) has recently been subjected to criticism and emphasis in relation to clinical efficacy and toxicity. It is well-established that ozone is one of the most potent oxidants but its toxicity is due to the cumulative ozone dose that, day by day, elicits the formation of noxious products. This fact has established the dogma that ozone is always toxic and its medical application should be proscribed. However, ozone therapy is becoming very useful both on its own or applied in combination with orthodox medicine in a broad range of pathologies, so it is important to clarify this controversial issue. In order to understand the problem of the toxicity induced by ozone, the aim of the present study was to evaluate whether O2-O3 treatment could have any toxic effect on the human NIH3T3 fibroblast cell line. We found that O2-O3 did not induce toxicity by using the appropriate dose in contrast with the established dogma that ozone is always toxic and its medical application should be proscribed.

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